Bioreactor Control System (Benchtop)

The BIOne 1250 is a modular, flexible bioreactor control system designed for precise control of agitation, temperature, pH, and dissolved oxygen. It supports cell culture, cell-free, and microbial fermentation processes with both single-use and autoclavable bioreactors, and combines real-time monitoring, data trending, and configurable control with an intuitive 12.1″ (30 cm) capacitive touchscreen interface.

Key Features

• Precise bioreactor control — Agitation, temperature, pH, and dissolved oxygen
• Flexible process support — Batch, fed-batch, and perfusion processes
• Configurable operation — Open access with unlocked control loops, configurable PIDs, cascade visualization, and customizable recipes
• Preloaded, subscription-free software — Data trending, automation, and recipe control; optional OPC for remote access via browser, Python, or SCADA
• PAT integration — Seamlessly integrates with advanced process analytical technologies for real-time monitoring and data-driven optimization
• Tech transfer ready — Characterized based on tip speed, mixing time, P/V, OTR, and k_La

Bioreactor Control System Specifications

The BIOne 1250 Dual allows simultaneous, independent control of two bioreactor or fermentation systems from a single central tower. With a compact 18″ (W) × 22″ (H) × 27″ (D) footprint, it supports both single-use and autoclavable vessels and carries the same intuitive interface and configurable control as the BIOne 1250.

Key Features

• Dual independent control — Simultaneous control of two independent bioreactor or fermentation systems from a single central tower
• Compact footprint — 18″ (W) × 22″ (H) × 27″ (D)
• 8 peristaltic pumps — 4 variable-speed pumps per side to handle even the most complex process addition strategies
• Expanded I/O — 8 analog inputs, 8 analog outputs, 4 digital inputs, 4 digital outputs for PAT integration
• Preloaded, subscription-free software — Data trending, automation, and recipe control; optional OPC for remote access via browser, Python, or SCADA
• PAT integration — Seamlessly integrates with advanced process analytical technologies for real-time monitoring and data-driven optimization

Bioreactor Control System Specifications

The BIOne 250 combines advanced functionality with cost-effectiveness, making it an ideal choice for laboratories and research facilities focused on microbial bioprocessing. Derived from the BIOne 1250, it offers an intuitive interface and remote control capabilities, enables seamless scalability, and is compatible with a range of bioprocessing equipment. Compatible with autoclavable glass (0.75L–10L) and single-use vessels (0.9L–10L).

Key Features

• Budget friendly — Advanced bioreactor control for microbial applications at a budget friendly price
• Remote operation — Remote control via PC, laptop, or external monitor (touchscreen or wired with mouse); monitoring and control from any web-enabled device
• Intuitive interface — Feature-rich interface derived from the BIOne 1250, designed to keep focus on the science, not the setup
• Gas control — 1 or 2 configurable rotameters; configurations include one rotameter and one gas, one rotameter and two gases, or two rotameters and two gases
• Three fixed-speed pumps — With option to add an external pump via additional I/O
• Integrated storage — Integrated shelving designed for efficient storage of media bottles, reagent bottles, and more
• Over 30 days of local data storage and trending

Bioreactor Control System Specifications

The BIOne Single-Use Bioreactor (SUB) is assembled in Distek’s ISO 7 cleanroom with USP Class VI, animal derivative-free materials, gamma sterilized, and ready to use out of the box — no cleaning, assembly, or autoclaving required. Available in 2L, 5L, and 10L. Well characterized based on k_La, P/V, and tip speed, simplifying the tech transfer and scale-up process.

Suited for monoclonal antibody production in CHO, AAV and lentiviral vectors in SF9 and HEK293, adherent and aggregate stem cells, and mRNA.

Key Features

• Ready-to-use — Gamma sterilized, assembled in Distek’s ISO 7 cleanroom with USP Class VI materials; no cleaning, assembly, or autoclaving required
• Animal derivative-free — Triple-layered low antioxidant liner: outer layer (LDPE), middle barrier layer (EVOH), contact layer (ULDPE)
• Agnostic design — Compatible with most bioprocessing controllers and most manufacturers’ glass vessels
• Configurable sensor connections — Standard headplate with ports for non-invasive DO (polarographic or optical), optional integrated single-use pH probe, optional non-invasive optical pH, and PG-13.5 universal sensor ports
• Configurable below the headplate — Single or dual pitched blade impellers; flute, microsparger, or open pipe sparger options
• Perfusion-ready — Compatible with Repligen® ATF perfusion tubing

BIOne SUB materials are rated for use in processes at temperatures up to 60°C (exception of Hamilton Single-Use pH probe, with a maximum operational temperature definition of 50°C). Structural Integrity Testing completed for operations up to 60°C. Leachable and Extractables Testing completed at 40°C.

Specifications

The BIOne Single-Use Fermentor (SUF) is a fully closed, ready-to-use system for demanding microbial applications including plasmid DNA production in E. coli and recombinant protein expression in yeast. Available in 2L and 10L. Assembled in Distek’s ISO 7 cleanroom with USP Class VI materials and gamma sterilized — no cleaning, assembly, or autoclave sterilization required.

Key Features

• Ready-to-use — Fully closed system; assembled in Distek’s ISO 7 cleanroom with USP Class VI materials, gamma sterilized; no cleaning, assembly, or sterilization required
• Optimized for high OTR — Dual Rushton impellers, baffle cage, maximum agitation rate of 1,250 RPM (2L SUF)
• Maximum gas exchange — High capacity sparge and exhaust filters; flute sparger with 7 × 1.5 mm holes
• Agnostic design — Compatible with most common bioprocessing controllers via a range of motor adapters
• Configurable sensor connections — Standard headplate with ports for non-invasive DO (polarographic), PG-13.5 universal sensor ports, and septum port; optional integrated single-use pH probe available
• Proven performance — E. coli growth profiles in the 2L SUF matched glass vessel controls in a 22-hour batch process under identical conditions

BIOne SUF materials are rated for use in processes at temperatures up to 60°C (exception of Hamilton Single-Use pH probe, rated to 50°C). Structural Integrity Testing completed for operations up to 60°C. Leachable and Extractables Testing completed at 40°C.

Specifications