Dissolved CO2 Sensor InPro 5500i Varivent Type N

Dissolved CO2 Sensor InPro 5500i TriClamp 2"

Dissolved CO2 Sensor InPro 5500i D28/M42

Dissolved CO2 Sensor

Inline CO₂ Monitoring for Biopharma and Fermentation

METTLER TOLEDO offers two industry-leading inline dissolved CO₂ sensor solutions to meet the demands of bioprocess and beverage production environments. The membrane-based InPro 5000i provides accurate low-range carbon dioxide measurement using the proven Severinghaus principle, making it ideal for cell culture and fermentation processes. For broader range applications, the InPro 5500i dissolved CO₂ sensor uses optical measurement technology to deliver fast, drift-free CO₂ readings with minimal maintenance.

Both sensors are compatible with CIP/SIP processes and equipped with Intelligent Sensor Management (ISM^®) for predictive diagnostics and simplified calibration, helping improve process reliability and reduce downtime across R&D and production-scale operations.

METTLER TOLEDO Intelligent Sensor Management

InPro 5000i: Membrane-Based CO₂ Sensor for Biopharma

METTLER TOLEDO’s InPro 5000i is a membrane-based sensor designed for precise inline measurement of dissolved carbon dioxide in biopharmaceutical applications. It uses the Severinghaus principle of potentiometric CO₂ detection — an industry-standard approach trusted for over 55 years.

This sensor is ideal for:

• Monitoring pCO₂ in mammalian cell culture and microbial fermentation
• Real-time control of CO₂ levels in bioreactors and closed-loop systems
• Applications requiring sterile, low-maintenance operation

Key benefits include:

• Fully sterilizable: supports CIP, SIP, and autoclave workflows
• High surface-finish stainless steel body to reduce contamination risk
• Durable membrane with simplified service—replacement takes minutes
• Replaceable internal pH electrode for easy onsite maintenance
• ISM® digital platform for predictive diagnostics and integration

The InPro 5000i helps ensure gas-phase balance and product quality in critical upstream processes while minimizing downtime and maintenance costs.

InPro 5500i Series: Dissolved CO₂ Sensor with ISM Technology

Dissolved CO2 Sensor InPro 5500i Varivent Type N

The InPro 5500i is a digital, optical dissolved CO₂ sensor designed for inline or immersion applications in biopharmaceutical, fermentation, and food & beverage processes. Built with METTLER TOLEDO’s Intelligent Sensor Management (ISM^®) platform, this sensor series offers real-time CO₂ monitoring with predictive diagnostics and reduced maintenance.

The 5500i family includes three model variants optimized for different installation needs:

• InPro 5500i/120/225: For immersion applications (e.g. open vessels, bypass loops)
• InPro 5500i/225/425: Inline installation with extended reach
• InPro 5500i/325/425: Inline installation with medium-length probe

All models deliver:

• Wide CO₂ detection range: 0 – 20% vol (up to 200,000 ppm)
• No electrolyte or recalibration required
• Fast, drift-free measurement via optical fluorescence quenching
• Digital ISM^® interface for plug-and-measure simplicity and process reliability

The InPro 5500i is also ideal for food and beverage applications such as carbonation control in beer and soft drink production, where fast response and hygienic design are essential. With compatibility for sanitary process connections, this sensor supports clean-in-place (CIP) and sterilize-in-place (SIP) procedures—making it a strong fit for both regulatory compliance and process consistency.

This series is widely used for bioreactor control, cell culture monitoring, fermentation optimization, and inline CO₂ monitoring across R&D, manufacturing, and beverage production environments.

Specifications – InPro 5500i (Var. Type N)

Note: The InPro 5500i is available in multiple process connection styles to suit different installation environments. All variants share the same measurement performance and ISM
diagnostics platform:

Tri-Clamp 2″ – sanitary clamp fitting
D28/M42 – 28 mm diameter with threaded cap nut
Varivent Type N – designed for Varivent process housings (shown below)

Measurement Parameter	Carbon Dioxide
Measurement Range	0…10 bar pCO₂, 0…15 g/L CO₂, 0…7 V/V CO₂
Measurement Range (PSI)	0…145 psig pCO₂
Accuracy	± 1% at ±5 °C of calibration temp; ±2% over 0–50 °C (32–122 °F)
Response Time	21 s (measurement cycle time)
Measurement Cycle	< 20 s
Measurement Technology	Thermal Conductivity
Temperature Compensation	Automatic with built-in RTD
Primary Media/Application	Food and Beverage
ISM (Digital) or Analog	ISM (Digital)
Digital Integration	RS485 – ISM
Wetted Metallic Material	316L stainless steel
Surface Roughness	N5/Ra16 (Ra = 0.4 μm / 16 μin)
O-Ring Material	EPDM
Wetted Membrane Material	Silicone
Flow Requirements	> 0.5 m/s
Autoclavable/Sterilizable	Sterilizable Only
CIP Resistant	Yes
IP Rating	IP 67
Hygienic/Biocompatibility Approvals	FDA
Certificates & Approvals	Quality certificate, Material certificate 3.1, Surface finish certificate
Measuring Temp. Range	32 °F – 122 °F (0 °C – 50 °C)
Mechanical Temp. Range	23 °F – 250 °F (-5 °C – 121 °C)
Operating Pressure (Bar)	0–20 bar
Operating Pressure (PSI)	Up to 362.6 psi
Design Pressure	290 psi (20 bar)
Connector Design	Straight
Cable Connector	M12, 5-pin
Process Connection	Varivent Type N
Model Family	InPro 5500i

What exactly is ISM?

With Mettler-Toledo’s advanced Intelligent Sensor Management (ISM), these concerns are a thing of the past! ISM has been around for years helping all kinds of processes have accurate and assured calibrations and maintenance statuses throughout the whole line of Mettler-Toledo sensors. This technology sends you automatic diagnostic data about your sensor’s:

• Health • Dynamic lifetime • Calibration quality
• Glass strength • Sensor drift

Utilizing this technology would allow you to go into a run or experiment 100% certain that your probes will not be an issue and can move forward with peace of mind. By choosing one of the many Mettler-Toledo sensors equipped with ISM technology, you’ll save on manual labor, experimental waste/time, and be sure you won’t have experimental error due to sensor drift or misreading. With all of this, it makes it an easy choice to move towards ISM probes for your R&D lab or large-scale facility.

Which brings us to a really BIG announcement…

Mettler-Toledo recently launched ISM 2.0 which comes equipped with the same features described but with greater control, reliability, and software to bolster the ease of use and peace of mind that comes with these sensors. ISM 2.0 adds upgrades to the lifetime and calibration algorithms that make them even more accurate at telling you the health and quality of your data and sensors. The upgrade comes partnered with more robust software for enhanced process reliability.